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KMID : 0545120110210030274
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 3 p.274 ~ p.276
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A RAPD-PCR Method for the Rapid Detection of Bacillus cereus
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Lee Ji-Yeon
Kwon Gun-Hee
Park Jae-Yong
Park Cheon-Seok
Kwon Dae-Young
Lim Jin-Kyu
Kim Jong-Sang
Kim Jeong-Hwan
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Abstract
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Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than 10(2)/g sample.
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KEYWORD
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Bacillus cereus, food poisoning, rapid detection, RAPD-PCR
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